Please suggest if we can monitor the traffic for these ports 80, 5009 using NTA. if not possible through NTA, please suggest tool which can help me in doing the same.
You can use the Flow Navigator on the NTA summary page to select specific applications by name or port number, and filter your view based on that selection criteria.
You can also alert for traffic volumes with specific applications / ports using the Alerting feature. See these two articles:
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